The Threatened Species Initiative is a national initiative whose objective is to create a national library of genomics data to support decision-making for biodiversity conservation. In collaboration with Bioplatforms Australia, Australian Department of Agriculture, Water and Environment (DAWE) and the WA Department of Biodiversity Conservation and Attractions, we are generating genome resources and associated population data, and developing an online toolkit to assist non-geneticists in analysing and interpreting their genetic data for conservation action. The program brings together genome biologists, population biologists, bioinformaticians, population geneticists and zoologists with conservation agencies across Australia, including government, zoos and NGOs.